Reaction participants Show >> << Hide
- Name help_outline (24R,241R)-fucosterol epoxide Identifier CHEBI:15577 (Beilstein: 4712006) help_outline Charge 0 Formula C29H48O2 InChIKeyhelp_outline XUBCLKHKJZCPFZ-UMKPQZCOSA-N SMILEShelp_outline [H][C@@]1(CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CC[C@@]1(O[C@@H]1C)C(C)C 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline desmosterol Identifier CHEBI:17737 (CAS: 313-04-2) help_outline Charge 0 Formula C27H44O InChIKeyhelp_outline AVSXSVCZWQODGV-DPAQBDIFSA-N SMILEShelp_outline [H][C@@]1(CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCC=C(C)C 2D coordinates Mol file for the small molecule Search links Involved in 6 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline acetaldehyde Identifier CHEBI:15343 (CAS: 75-07-0) help_outline Charge 0 Formula C2H4O InChIKeyhelp_outline IKHGUXGNUITLKF-UHFFFAOYSA-N SMILEShelp_outline [H]C(C)=O 2D coordinates Mol file for the small molecule Search links Involved in 50 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:10884 | RHEA:10885 | RHEA:10886 | RHEA:10887 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Fucosterol epoxide lyase of insects: synthesis of labeled substrates and development of a partition assay.
Prestwich G.D., Angelastro M., De Palma A., Perino M.A.
Fucosterol epoxide labeled with tritium in the C-29 methyl has been synthesized and employed in the development of a partition assay which allows the rapid determination of fucosterol epoxide lyase activity in vitro in homogenates of insect tissues. An independent synthesis of [24-14C]fucosterol e ... >> More
Fucosterol epoxide labeled with tritium in the C-29 methyl has been synthesized and employed in the development of a partition assay which allows the rapid determination of fucosterol epoxide lyase activity in vitro in homogenates of insect tissues. An independent synthesis of [24-14C]fucosterol epoxide provided a control substrate to evaluate nondealkylative transfer of labeled steroid to the aqueous layer during the enzyme assay. The diastereomeric 24R,28R- and 24S,28S-[29-3H]fucosterol epoxides were obtained via HPLC separation of their benzoate esters. Homogenates of the midgut tissue of larval tobacco hornworms (Manduca sexta) were examined at pH 5 to 9 in several buffer systems, and at temperatures of 7 to 67 degrees C in phosphate buffer. Optimal activity was found using pH 7.4, 76 mM phosphate buffer at 37 degrees C. The 24R,28R diastereomer of fucosterol epoxide was metabolized at a rate at least 100 times that of the 24S,28S isomer by this enzyme system. << Less