Enzymes
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- Name help_outline a nucleoside 5'-phosphate Identifier CHEBI:57867 Charge -2 Formula C5H7O6PR2 SMILEShelp_outline O[C@H]1[C@@H]([*])[C@H]([*])O[C@@H]1COP([O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 210 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline ATP Identifier CHEBI:30616 (Beilstein: 3581767) help_outline Charge -4 Formula C10H12N5O13P3 InChIKeyhelp_outline ZKHQWZAMYRWXGA-KQYNXXCUSA-J SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,256 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline a 5'-phosphonucleoside 3'-diphosphate Identifier CHEBI:144554 Charge -5 Formula C5H6O12P3R2 SMILEShelp_outline [C@H]1([C@H](O[C@H]([C@@H]1*)*)COP([O-])([O-])=O)OP(OP([O-])([O-])=O)([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline AMP Identifier CHEBI:456215 Charge -2 Formula C10H12N5O7P InChIKeyhelp_outline UDMBCSSLTHHNCD-KQYNXXCUSA-L SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 487 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:12713 | RHEA:12714 | RHEA:12715 | RHEA:12716 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Streptomyces ATP nucleotide 3'-pyrophosphokinase-gene cloning and sequence analysis.
Higuchi T., Mikuniya T., Osoegawa K., Ezaki S., Sumichika H., Mizui Y., Shoji T., Kishihara K., Muta S., Kuhara S. , et al.
Streptomyces ATP nucleotide 3'-pyrophosphokinase is an extracellular enzyme that transfers 5'-beta, gamma-pyrophosphoryl groups of ATP to a variety of nucleotides at the 3'-OH site. The enzyme gene was cloned from partially Sau3AI-digested chromosomal DNA of S. morookaensis in S. lividans TK24/pIJ ... >> More
Streptomyces ATP nucleotide 3'-pyrophosphokinase is an extracellular enzyme that transfers 5'-beta, gamma-pyrophosphoryl groups of ATP to a variety of nucleotides at the 3'-OH site. The enzyme gene was cloned from partially Sau3AI-digested chromosomal DNA of S. morookaensis in S. lividans TK24/pIJ699 and then in E. coli JM83/pUC12. Some transformants produced the active enzyme. The gene was sequenced by the dideoxynucleotide termination procedure. Its GC content was 72%. Its putative promoter regions, showing little homology to that of the Streptomyces consensus type, were pointed out. No sequence homology was found between the pyrophosphokinase and any other known genes including those of the most mechanistically similar bacterial stringent factor and related proteins. Northern hybridization analysis showed that the gene is constitutionally polycistronic and expressed under transcriptional control. Nuclease S1 mapping indicated that the gene transcription starts from its translation initiation site. << Less
Biosci Biotechnol Biochem 58:2182-2187(1994) [PubMed] [EuropePMC]
Comments
Published in: "Isolation and identification of ATP:nucleotide pyrophosphotransferase-producing microorganism." Murao, S. and Nishino, T. Agric. Biol. Chem. 38 (1974) 2483–2489 "Purification and some properties of ATP:nucleotide pyrophosphotransferase of Streptomyces adephospholyticus." Nishino, T. and Murao, S. Agric. Biol. Chem. 38 (1974) 2491–2496 "Characterization of pyrophosphoryl transfer reaction of ATP:nucleotide pyrophosphotransferase." Nishino, T. and Murao, S. Agric. Biol. Chem. 39 (1975) 1007–1014.