Enzymes
| UniProtKB help_outline | 4 proteins |
| Enzyme class help_outline |
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| GO Molecular Function help_outline |
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- Name help_outline a 1,2-diacyl-sn-glycerol Identifier CHEBI:17815 Charge 0 Formula C5H6O5R2 SMILEShelp_outline OC[C@@H](COC([*])=O)OC([*])=O 2D coordinates Mol file for the small molecule Search links Involved in 184 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline a glycerophospholipid Identifier CHEBI:136912 Charge -1 Formula C5H5O8PR3 SMILEShelp_outline *OP(=O)([O-])OC[C@H](OC(*)=O)COC(=O)* 2D coordinates Mol file for the small molecule Search links Involved in 8 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline a monoacylglycerophospholipid Identifier CHEBI:136913 Charge -1 Formula C4H6O7PR2 SMILEShelp_outline *OP(=O)([O-])OC[C@H](O*)CO* 2D coordinates Mol file for the small molecule Search links Involved in 3 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline a triacyl-sn-glycerol Identifier CHEBI:64615 Charge 0 Formula C6H5O6R3 SMILEShelp_outline [*]C(=O)OC[C@H](COC([*])=O)OC([*])=O 2D coordinates Mol file for the small molecule Search links Involved in 32 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:14057 | RHEA:14058 | RHEA:14059 | RHEA:14060 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants.
Dahlqvist A., Stahl U., Lenman M., Banas A., Lee M., Sandager L., Ronne H., Stymne S.
Triacylglycerol (TAG) is known to be synthesized in a reaction that uses acyl-CoA as acyl donor and diacylglycerol (DAG) as acceptor, and which is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase. We have found that some plants and yeast also have an acyl-CoA-independent mechanism f ... >> More
Triacylglycerol (TAG) is known to be synthesized in a reaction that uses acyl-CoA as acyl donor and diacylglycerol (DAG) as acceptor, and which is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase. We have found that some plants and yeast also have an acyl-CoA-independent mechanism for TAG synthesis, which uses phospholipids as acyl donors and DAG as acceptor. This reaction is catalyzed by an enzyme that we call phospholipid:diacylglycerol acyltransferase, or PDAT. PDAT was characterized in microsomal preparations from three different oil seeds: sunflower, castor bean, and Crepis palaestina. We found that the specificity of the enzyme for the acyl group in the phospholipid varies between these species. Thus, C. palaestina PDAT preferentially incorporates vernoloyl groups into TAG, whereas PDAT from castor bean incorporates both ricinoleoyl and vernoloyl groups. We further found that PDAT activity also is present in yeast microsomes. The substrate specificity of this PDAT depends on the head group of the acyl donor, the acyl group transferred, and the acyl chains of the acceptor DAG. The gene encoding the enzyme was identified. The encoded PDAT protein is related to lecithin:cholesterol acyltransferase, which catalyzes the acyl-CoA-independent synthesis of cholesterol esters. However, budding yeast PDAT and its relatives in fission yeast and Arabidopsis form a distinct branch within this protein superfamily, indicating that a separate PDAT enzyme arose at an early point in evolution. << Less
Proc. Natl. Acad. Sci. U.S.A. 97:6487-6492(2000) [PubMed] [EuropePMC]
This publication is cited by 3 other entries.