Enzymes
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Reaction participants Show >> << Hide
- Name help_outline 2,4-dichloro-5-oxo-2,5-dihydro-2-furylacetate Identifier CHEBI:57641 Charge -1 Formula C6H3Cl2O4 InChIKeyhelp_outline RNYNGUYSDYOCLB-UHFFFAOYSA-M SMILEShelp_outline [O-]C(=O)CC1(Cl)OC(=O)C(Cl)=C1 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline (2E,4E)-2,4-dichloromuconate Identifier CHEBI:11438 Charge -2 Formula C6H2Cl2O4 InChIKeyhelp_outline FHXOPKKNGKBBKG-DXLKSGPOSA-L SMILEShelp_outline [O-]C(=O)\C=C(Cl)/C=C(/Cl)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:17437 | RHEA:17438 | RHEA:17439 | RHEA:17440 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Purification and characterization of dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134.
Kuhm A.E., Schlomann M., Knackmuss H.J., Pieper D.H.
Dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134 was purified to homogeneity. The enzyme has an Mr of about 270,000 as determined by gel filtration and consists of six to eight subunits of identical Mr 40,000 as determined by SDS/PAGE. Mn2+ ions as well as thiol groups are requir ... >> More
Dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134 was purified to homogeneity. The enzyme has an Mr of about 270,000 as determined by gel filtration and consists of six to eight subunits of identical Mr 40,000 as determined by SDS/PAGE. Mn2+ ions as well as thiol groups are required for activity. A high Km value of about 4 mM for cis,cis-muconate explains the reported low activity with this compound. Relatively high Km values were also calculated for monochloro-substituted cis,cis-muconates (300-500 microM), in contrast with the low Km value of 20 microM for 2,4-dichloro-cis,cis-muconate. The catalytic constant of the pure enzyme was 3820 min-1 when measured with 2,4-dichloro-cis,cis-muconate. << Less