Enzymes
| Enzyme class help_outline |
|
| GO Molecular Function help_outline |
|
Reaction participants Show >> << Hide
- Name help_outline D-hamamelose Identifier CHEBI:18004 (CAS: 4573-78-8) help_outline Charge 0 Formula C6H12O6 InChIKeyhelp_outline ZGVNGXVNRCEBDS-HSUXUTPPSA-N SMILEShelp_outline [H]C(=O)[C@@](O)(CO)[C@H](O)[C@H](O)CO 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline ATP Identifier CHEBI:30616 (Beilstein: 3581767) help_outline Charge -4 Formula C10H12N5O13P3 InChIKeyhelp_outline ZKHQWZAMYRWXGA-KQYNXXCUSA-J SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,284 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline D-hamamelose 2'-phosphate Identifier CHEBI:57828 Charge -2 Formula C6H11O9P InChIKeyhelp_outline RIOZVCDMYGAYCJ-HSUXUTPPSA-L SMILEShelp_outline [H]C(=O)[C@@](O)(COP([O-])([O-])=O)[C@H](O)[C@H](O)CO 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline ADP Identifier CHEBI:456216 (Beilstein: 3783669) help_outline Charge -3 Formula C10H12N5O10P2 InChIKeyhelp_outline XTWYTFMLZFPYCI-KQYNXXCUSA-K SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 841 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,521 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:22796 | RHEA:22797 | RHEA:22798 | RHEA:22799 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
|
|||
| EC numbers help_outline | ||||
| Gene Ontology help_outline | ||||
| KEGG help_outline | ||||
| MetaCyc help_outline |
Publications
-
Purification and properties of hamamelosekinase.
Beck E., Wieczorek J., Reinecke W.
Hamamelosekinase (ATP:hamamelose 2(1)-phosphotransferase) was purified from a crude extract of Kluyvera citrophila 627 (Enterobacteriaeceae) which has been grown on D-hamamelose. Ammonium-sulfate fractionation and twofold chromatography on DEAE-cellulose resulted in a 51-fold purification of the e ... >> More
Hamamelosekinase (ATP:hamamelose 2(1)-phosphotransferase) was purified from a crude extract of Kluyvera citrophila 627 (Enterobacteriaeceae) which has been grown on D-hamamelose. Ammonium-sulfate fractionation and twofold chromatography on DEAE-cellulose resulted in a 51-fold purification of the enzyme. Neither glucosekinase nor significant ATPase activity could be detected in the pure preparation. Besides D-hamamelose only D-hamamelitol was utilized as a substrate; however, the latter was phosphorylated at a very low rate. The molecular weight of the enzyme as estimated by gel chromatography is 21 000. The Km values for hamamelose and ATP were 3 mM nd 2.5 mM, respectively. The pH optimum was found at 7.5. In contrast to hexokinase, purified hamamelosekinase is very labile and could only be stabilized by addition of its substrate D-hamamelose. The most unusual property with respect to yeast hexokinase is a pronounced substrate inhibiton by hamamelose (> 5mM) and ATP (> 7mM), respectively, which could be interpreted as due to an economic utilization of the nutrient. Hamamelosekinase as well as glucosekinase are inducible by growing the microorganisms on the corresponding monosaccharides. << Less