Enzymes
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- Name help_outline (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate Identifier CHEBI:57409 Charge -1 Formula C20H31O3 InChIKeyhelp_outline JSFATNQSLKRBCI-VAEKSGALSA-M SMILEShelp_outline CCCCC[C@H](O)\C=C\C=C/C\C=C/C\C=C/CCCC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 6 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline NADP+ Identifier CHEBI:58349 Charge -3 Formula C21H25N7O17P3 InChIKeyhelp_outline XJLXINKUBYWONI-NNYOXOHSSA-K SMILEShelp_outline NC(=O)c1ccc[n+](c1)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,262 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate Identifier CHEBI:57410 Charge -1 Formula C20H29O3 InChIKeyhelp_outline YGJTUEISKATQSM-USWFWKISSA-M SMILEShelp_outline CCCCCC(=O)\C=C\C=C/C\C=C/C\C=C/CCCC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,331 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline NADPH Identifier CHEBI:57783 (Beilstein: 10411862) help_outline Charge -4 Formula C21H26N7O17P3 InChIKeyhelp_outline ACFIXJIJDZMPPO-NNYOXOHSSA-J SMILEShelp_outline NC(=O)C1=CN(C=CC1)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,256 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:23264 | RHEA:23265 | RHEA:23266 | RHEA:23267 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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15-Hydroxyeicosatetraenoic acid dehydrogenase activity in microsomal fraction of mouse liver homogenate.
Sok D.E., Kang J.B., Shin H.D.
The microsomal fraction of mouse liver homogenate showed NAD(P)-dependent dehydrogenase activity involved in the conversion of 15-hydroxyeicosatetraenoic acid to 15-ketoeicosatetraenoic acid, which was determined quantitatively by HPLC assay. This enzyme, tightly bound to membranes and relatively ... >> More
The microsomal fraction of mouse liver homogenate showed NAD(P)-dependent dehydrogenase activity involved in the conversion of 15-hydroxyeicosatetraenoic acid to 15-ketoeicosatetraenoic acid, which was determined quantitatively by HPLC assay. This enzyme, tightly bound to membranes and relatively stable, possessed apparent values of Km of 8.3 microM and Vmax of 2.8 nmoles/mg.min in the oxidation of 15-HETE, and gave an optimum pH of 9.8. Additionally, the enzyme, not susceptible to the inhibition by indomethacin and showing a similar cosubstrate specificity between NAD and NADP, utilized other hydroxylated eicosanoids as substrates, based on HPLC analyses. << Less
Biochem Biophys Res Commun 156:524-529(1988) [PubMed] [EuropePMC]