Reaction participants Show >> << Hide
- Name help_outline sucrose Identifier CHEBI:17992 (Beilstein: 90825; CAS: 57-50-1) help_outline Charge 0 Formula C12H22O11 InChIKeyhelp_outline CZMRCDWAGMRECN-UGDNZRGBSA-N SMILEShelp_outline OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 27 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 6-O-α-D-glucopyranosyl-D-fructose Identifier CHEBI:18394 (Beilstein: 1435314; CAS: 13718-94-0,15132-06-6) help_outline Charge 0 Formula C12H22O11 InChIKeyhelp_outline PVXPPJIGRGXGCY-TZLCEDOOSA-N SMILEShelp_outline OC[C@H]1O[C@H](OC[C@H]2OC(O)(CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:24032 | RHEA:24033 | RHEA:24034 | RHEA:24035 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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The extraction and mechanism of a novel isomaltulose-synthesizing enzyme from Erwinia rhapontici.
Cheetham P.S.
The single enzyme that mediates the bioconversion is demonstrated to be located in the cells' periplasmic space, a site that facilitates its use as an industrial biocatalyst, and to be a previously undescribed hexosyltransferase with four novel features. The enzyme is sucrose-specific, and has an ... >> More
The single enzyme that mediates the bioconversion is demonstrated to be located in the cells' periplasmic space, a site that facilitates its use as an industrial biocatalyst, and to be a previously undescribed hexosyltransferase with four novel features. The enzyme is sucrose-specific, and has an intramolecular mechanism in which both glucose and fructose residues appear to be enzyme-bound. Thirdly, it is reaction-non-selective, forming simultaneously isomaltulose and a second hitherto uncharacterized alpha-(1----1)-linked disaccharide (trehalulose), by hydrolysis of sucrose followed by reaction of glucose with the C-6 and C-1 positions of the fructofuranose respectively. Finally, on extended incubation an unusual recycling mechanism caused the concentration of isomaltulose, the kinetically preferred product, to reach a transient maximum concentration and then fall, and the concentration of trehalulose, the thermodynamically favoured product, to rise slowly. << Less