Enzymes
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Reaction participants Show >> << Hide
- Name help_outline 8'-apo-β-carotenol Identifier CHEBI:18092 (Beilstein: 2482406) help_outline Charge 0 Formula C30H42O InChIKeyhelp_outline YKSARTKNUYWHKA-DOKBYWHISA-N SMILEShelp_outline C\C(CO)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline O2 Identifier CHEBI:15379 (CAS: 7782-44-7) help_outline Charge 0 Formula O2 InChIKeyhelp_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILEShelp_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,648 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline (3E,5E)-7-hydroxy-6-methylhepta-3,5-dienal Identifier CHEBI:51922 Charge 0 Formula C8H12O2 InChIKeyhelp_outline ARBHCEGTLUXZRX-YNRRLODASA-N SMILEShelp_outline C\C(CO)=C/C=C/CC=O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 14'-apo-β-carotenal Identifier CHEBI:17457 (Beilstein: 2056663; CAS: 6985-27-9) help_outline Charge 0 Formula C22H30O InChIKeyhelp_outline NGISIFNAHMKVQR-SSRYJDFZSA-N SMILEShelp_outline [H]C(=O)\C=C\C=C(C)\C=C\C=C(C)\C=C\C1=C(C)CCCC1(C)C 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:26023 | RHEA:26024 | RHEA:26025 | RHEA:26026 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Enzymatic oxidation of beta-apo-8'-carotenol to beta-apo-14'-carotenal by an enzyme different from beta-carotene-15,15'-dioxygenase.
Dmitrovskii A.A., Gessler N.N., Gomboeva S.B., Ershov YuV, Bykhovsky VYa
Extracts of rat and rabbit intestinal mucosa were fractionated with ammonium sulfate. The precipitate contained beta-apocarotenoid-14',13'-dioxygenase (ADO) activity. beta-Apo-14'-carotenal was found to be the product of enzymatic cleavage of beta-apo-8'-carotenol. Activities of ADO and beta-carot ... >> More
Extracts of rat and rabbit intestinal mucosa were fractionated with ammonium sulfate. The precipitate contained beta-apocarotenoid-14',13'-dioxygenase (ADO) activity. beta-Apo-14'-carotenal was found to be the product of enzymatic cleavage of beta-apo-8'-carotenol. Activities of ADO and beta-carotene-15,15'-dioxygenase (CDO) were detected in the presence of thiols and were inactivated by 1,10-phenanthroline. Optimal pH values were 7.0 for ADO and 8.0 for CDO. Heating at 52 degrees C inhibited ADO by 70% and produced no effect on CDO. ADO activity was maximal in the presence of sodium cholate or 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propanesulfonate (CHAPS). Sodium dodecylsulfate was required for maximal CDO activity. Proteins with ADO activity were not retained by phenyl-Sepharose CL-4B. CDO activity was eluted from columns only in the presence of detergents. The data suggest that the enzymes that catalyze the oxidative cleavage of beta-carotene to yield retinal are different from those that cleave beta-apo-8'-carotenol to yield beta-apo-14'-carotenal. << Less