Enzymes
| UniProtKB help_outline | 4,792 proteins |
Reaction participants Show >> << Hide
- Name help_outline fumarate Identifier CHEBI:29806 (Beilstein: 1861276; CAS: 142-42-7) help_outline Charge -2 Formula C4H2O4 InChIKeyhelp_outline VZCYOOQTPOCHFL-OWOJBTEDSA-L SMILEShelp_outline [O-]C(=O)\C=C\C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 40 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline succinate Identifier CHEBI:30031 (Beilstein: 1863859; CAS: 56-14-4) help_outline Charge -2 Formula C4H4O4 InChIKeyhelp_outline KDYFGRWQOYBRFD-UHFFFAOYSA-L SMILEShelp_outline [O-]C(=O)CCC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 325 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:29323 | RHEA:29324 | RHEA:29325 | RHEA:29326 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
|
|||
| MetaCyc help_outline | ||||
| EcoCyc help_outline |
Publications
-
DcuA of aerobically grown Escherichia coli serves as a nitrogen shuttle (L-aspartate/fumarate) for nitrogen uptake.
Strecker A., Schubert C., Zedler S., Steinmetz P., Unden G.
DcuA of Escherichia coli is known as an alternative C<sub>4</sub> -dicarboxylate transporter for the main anaerobic C<sub>4</sub> -dicarboxylate transporter DcuB. Since dcuA is expressed constitutively under aerobic and anaerobic conditions, DcuA was suggested to serve aerobically as a backup for ... >> More
DcuA of Escherichia coli is known as an alternative C<sub>4</sub> -dicarboxylate transporter for the main anaerobic C<sub>4</sub> -dicarboxylate transporter DcuB. Since dcuA is expressed constitutively under aerobic and anaerobic conditions, DcuA was suggested to serve aerobically as a backup for the aerobic (DctA) transporter, or for the anabolic uptake of C<sub>4</sub> -dicarboxylates. In this work, it is shown that DcuA is required for aerobic growth with L-aspartate as a nitrogen source, whereas for growth with L-aspartate as a carbon source, DctA was needed. Strains with DcuA catalyzed L-aspartate and C<sub>4</sub> -dicarboxylate uptake (like DctA), or an L-aspartate/C<sub>4</sub> -dicarboxylate antiport (unlike DctA). DcuA preferred L-aspartate to succinate in transport (K<sub>M</sub> = 43 and 844 µM, respectively), whereas DctA has higher affinity for C<sub>4</sub> -dicarboxylates like succinate compared to L-aspartate. When L-aspartate was supplied as the sole nitrogen source together with glycerol as the carbon source, L-aspartate was taken up by the bacteria and fumarate (or L-malate) was excreted in equimolar amounts. Both reactions depended on DcuA. L-Aspartate was taken up in amounts required for nitrogen metabolism but not for carbon metabolism. Therefore, DcuA catalyzes an L-aspartate/C<sub>4</sub> -dicarboxylate antiport serving as a nitrogen shuttle for nitrogen supply without net carbon supply. << Less
Mol. Microbiol. 109:801-811(2018) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.