Rhea - reaction knowledgebase

Enzymes

UniProtKB ^help_outline	3,683 proteins
Enzyme class ^help_outline	EC 2.4.1.256 dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase
GO Molecular Function ^help_outline	GO:0106073 dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity

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Name ^help_outline dolichyl β-D-glucosyl phosphate Identifier CHEBI:57525 Charge -1 Formula C26H46O9P(C5H8)n Search links Involved in 6 reaction(s) Find proteins in UniProtKB for this molecule Form(s) in this reaction:
- Identifier: RHEA-COMP:9528
  
  Polymer name: a dolichyl β-D-glucosyl phosphate
  
  Polymerization index ^help_outline n
  
  Formula C26H46O9P(C5H8)_n
  
  Charge (-1)(0)_n
  Mol File for the polymer
Name ^help_outline α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol Identifier CHEBI:132522 Charge -2 Formula (C5H8)n.C102H172N2O72P2 Search links Involved in 2 reaction(s) Find proteins in UniProtKB for this molecule Form(s) in this reaction:
- Identifier: RHEA-COMP:12633
  
  Polymer name: α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol
  
  Polymerization index ^help_outline n
  
  Formula C102H172N2O72P2(C5H8)_n
  
  Charge (-2)(0)_n
  Mol File for the polymer
Name ^help_outline dolichyl phosphate Identifier CHEBI:57683 Charge -2 Formula C20H35O4P(C5H8)n Search links Involved in 24 reaction(s) Find proteins in UniProtKB for this molecule Form(s) in this reaction:
- Identifier: RHEA-COMP:9517
  
  Polymer name: a dolichyl phosphate
  
  Polymerization index ^help_outline n
  
  Formula C20H35O4P(C5H8)_n
  
  Charge (-2)(0)_n
  Mol File for the polymer
Name ^help_outline α-D-Glc-(1→2)-α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol Identifier CHEBI:132523 Charge -2 Formula (C5H8)n.C108H182N2O77P2 Search links Involved in 2 reaction(s) Find proteins in UniProtKB for this molecule Form(s) in this reaction:
- Identifier: RHEA-COMP:12634
  
  Polymer name: α-D-Glc-(1→2)-α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol
  
  Polymerization index ^help_outline n
  
  Formula C108H182N2O77P2(C5H8)_n
  
  Charge (-2)(0)_n
  Mol File for the polymer
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:29543	RHEA:29544	RHEA:29545	RHEA:29546
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	3,683 proteins
EC numbers ^help_outline	2.4.1.256
Gene Ontology ^help_outline	GO:0106073
KEGG ^help_outline				R06264
MetaCyc ^help_outline		RXN-5472

Publications

The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation.

Burda P., Aebi M.

The biosynthesis of the lipid-linked oligosaccharide substrate for N-linked protein glycosylation follows a highly conserved pathway at the membrane of the endoplasmic reticulum. Based on the synthetic growth defect in combination with a reduced oligosaccharyltransferase activity (wbp1), we have i ... >> More

The biosynthesis of the lipid-linked oligosaccharide substrate for N-linked protein glycosylation follows a highly conserved pathway at the membrane of the endoplasmic reticulum. Based on the synthetic growth defect in combination with a reduced oligosaccharyltransferase activity (wbp1), we have identified alg10 mutant strains which accumulate lipid-linked Glc2Man9GlcNAc2. We cloned the corresponding wild-type gene and show in a novel in vitro assay that Alg10p is a dolichyl-phosphoglucose-dependent glucosyltransferase which adds the terminal alpha-1,2 glucose to the lipid-linked Glc2Man9GlcNAc2 oligosaccharide. Hypoglycosylation of secreted proteins in alg10 deletion strains demonstrates that the terminal alpha-1,2-linked glucose residue is a key element in substrate recognition by the oligosaccharyltransferase. This ensures that primarily completely assembled oligosaccharide is transferred to protein. << Less

Glycobiology 8:455-462(1998) [PubMed] [EuropePMC]

Enzymes

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Cross-references

Publications

The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation.