Rhea - reaction knowledgebase

Enzymes

UniProtKB ^help_outline	2,641 proteins
Enzyme class ^help_outline	EC 2.8.1.14 tRNA-5-taurinomethyluridine 2-sulfurtransferase
GO Molecular Function ^help_outline	GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase

Reaction participants Show >> << Hide

Name^help_outline 5-taurinomethyluridine³⁴ in tRNA Identifier RHEA-COMP:11732 Reactive part ^help_outline
- Name ^help_outline 5-taurinomethyluridine 5'-phosphate residue Identifier CHEBI:87172 Charge -1 Formula C12H17N3O11PS Position^help_outline 34 SMILES^help_outline O[C@@H]1[C@H](O-*)[C@@H](COP([O-])(-*)=O)O[C@H]1n1cc(C[NH2+]CCS([O-])(=O)=O)c(=O)[nH]c1=O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 1 reaction(s) Find proteins in UniProtKB for this molecule
Name ^help_outline AH₂ Identifier CHEBI:17499 Charge 0 Formula RH2 SMILES^help_outline *([H])[H] 2D coordinates Mol file for the small molecule Search links Involved in 2,713 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline ATP Identifier CHEBI:30616 (Beilstein: 3581767) ^help_outline Charge -4 Formula C10H12N5O13P3 InChIKey^help_outline ZKHQWZAMYRWXGA-KQYNXXCUSA-J SMILES^help_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,256 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline S-sulfanyl-L-cysteinyl-[protein] Identifier RHEA-COMP:11726 Reactive part ^help_outline
- Name ^help_outline S-sulfanyl-L-cysteine residue Identifier CHEBI:61963 Charge 0 Formula C3H5NOS2 SMILES^help_outline C([C@H](CSS)N*)(=O)* 2D coordinates Mol file for the small molecule Search links Involved in 16 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 4 reaction(s) Find proteins in UniProtKB for this molecule
Name^help_outline 5-taurinomethyl-2-thiouridine³⁴ in tRNA Identifier RHEA-COMP:11733 Reactive part ^help_outline
- Name ^help_outline 5-taurinomethyl-2-thiouridine 5'-phosphate residue Identifier CHEBI:87171 Charge -1 Formula C12H17N3O10PS2 Position^help_outline 34 SMILES^help_outline O[C@@H]1[C@H](O-*)[C@@H](COP([O-])(-*)=O)O[C@H]1n1cc(C[NH2+]CCS([O-])(=O)=O)c(=O)[nH]c1=S 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 1 reaction(s) Find proteins in UniProtKB for this molecule
Name ^help_outline A Identifier CHEBI:13193 Charge Formula R SMILES^help_outline * 2D coordinates Mol file for the small molecule Search links Involved in 2,783 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline AMP Identifier CHEBI:456215 Charge -2 Formula C10H12N5O7P InChIKey^help_outline UDMBCSSLTHHNCD-KQYNXXCUSA-L SMILES^help_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 487 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline diphosphate Identifier CHEBI:33019 (Beilstein: 185088) ^help_outline Charge -3 Formula HO7P2 InChIKey^help_outline XPPKVPWEQAFLFU-UHFFFAOYSA-K SMILES^help_outline OP([O-])(=O)OP([O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 1,085 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name^help_outline L-cysteinyl-[protein] Identifier RHEA-COMP:10131 Reactive part ^help_outline
- Name ^help_outline L-cysteine residue Identifier CHEBI:29950 Charge 0 Formula C3H5NOS SMILES^help_outline C(=O)(*)[C@@H](N*)CS 2D coordinates Mol file for the small molecule Search links Involved in 123 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Search links Involved in 31 reaction(s) Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:47040	RHEA:47041	RHEA:47042	RHEA:47043
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	2,641 proteins
EC numbers ^help_outline	2.8.1.14
Gene Ontology ^help_outline	GO:0061708
MetaCyc ^help_outline		RXN-16821

Publications

Deletion of the MTO2 gene related to tRNA modification causes a failure in mitochondrial RNA metabolism in the yeast Saccharomyces cerevisiae.

Wang X., Yan Q., Guan M.X.

We report here the characterization of the yeast mto2 null mutants carrying wild-type mitochondrial DNA or 15S rRNA C1049G allele. The amounts of mitochondrial tRNA(Lys), tRNA(Glu), tRNA(Gln), tRNA(Leu), tRNA(Gly) and tRNA(Met) were markedly decreased but those of tRNA(Arg) and tRNA(His) were not ... >> More

We report here the characterization of the yeast mto2 null mutants carrying wild-type mitochondrial DNA or 15S rRNA C1049G allele. The amounts of mitochondrial tRNA(Lys), tRNA(Glu), tRNA(Gln), tRNA(Leu), tRNA(Gly) and tRNA(Met) were markedly decreased but those of tRNA(Arg) and tRNA(His) were not affected in mto2 strains. The mto2 strains exhibited significant reduction in the aminoacylation of tRNA(Lys), tRNA(Leu) but almost no effect in those of tRNA(His). Interestingly, the strain carrying the C1049G allele exhibited an impairment of aminoacylation of those tRNAs. Furthermore, the steady-state levels of mitochondrial mRNA CYTB, COX1, COX2, COX3, and ATP6 were markedly decreased in mto2 strains. These data strongly indicate that unmodified tRNA caused by the deletion of MTO2 caused the instability of mitochondrial tRNAs and mRNAs and impairment of aminoacylation of tRNAs. << Less

FEBS Lett. 581:4228-4234(2007) [PubMed] [EuropePMC]
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases.

Umeda N., Suzuki T., Yukawa M., Ohya Y., Shindo H., Watanabe K., Suzuki T.

Human mitochondrial (mt) tRNA(Lys) has a taurine-containing modified uridine, 5-taurinomethyl-2-thiouridine (taum5s2U), at its anticodon wobble position. We previously found that the mt tRNA(Lys), carrying the A8344G mutation from cells of patients with myoclonus epilepsy associated with ragged-re ... >> More

Human mitochondrial (mt) tRNA(Lys) has a taurine-containing modified uridine, 5-taurinomethyl-2-thiouridine (taum5s2U), at its anticodon wobble position. We previously found that the mt tRNA(Lys), carrying the A8344G mutation from cells of patients with myoclonus epilepsy associated with ragged-red fibers (MERRF), lacks the taum5s2U modification. Here we describe the identification and characterization of a tRNA-modifying enzyme MTU1 (mitochondrial tRNA-specific 2-thiouridylase 1) that is responsible for the 2-thiolation of the wobble position in human and yeast mt tRNAs. Disruption of the yeast MTU1 gene eliminated the 2-thio modification of mt tRNAs and impaired mitochondrial protein synthesis, which led to reduced respiratory activity. Furthermore, when MTO1 or MSS1, which are responsible for the C5 substituent of the modified uridine, was disrupted along with MTU1, a much more severe reduction in mitochondrial activity was observed. Thus, the C5 and 2-thio modifications act synergistically in promoting efficient cognate codon decoding. Partial inactivation of MTU1 in HeLa cells by small interference RNA also reduced their oxygen consumption and resulted in mitochondria with defective membrane potentials, which are similar phenotypic features observed in MERRF. << Less

J. Biol. Chem. 280:1613-1624(2005) [PubMed] [EuropePMC]

RHEA:47040 5-taurinomethyluridine(34) in tRNA + AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] = 5-taurinomethyl-2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]

Enzymes

Reaction participants Show >> << Hide

Cross-references

Publications

Deletion of the MTO2 gene related to tRNA modification causes a failure in mitochondrial RNA metabolism in the yeast Saccharomyces cerevisiae.

Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases.