Reaction participants Show >> << Hide
- Name help_outline acetate Identifier CHEBI:30089 (CAS: 71-50-1) help_outline Charge -1 Formula C2H3O2 InChIKeyhelp_outline QTBSBXVTEAMEQO-UHFFFAOYSA-M SMILEShelp_outline CC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 186 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline ATP Identifier CHEBI:30616 (Beilstein: 3581767) help_outline Charge -4 Formula C10H12N5O13P3 InChIKeyhelp_outline ZKHQWZAMYRWXGA-KQYNXXCUSA-J SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,355 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 10,232 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline acetyl-AMP Identifier CHEBI:142453 Charge -1 Formula C12H15N5O8P InChIKeyhelp_outline UBPVOHPZRZIJHM-WOUKDFQISA-M SMILEShelp_outline NC1=NC=NC2=C1N=CN2[C@@H]3O[C@H](COP(=O)(OC(C)=O)[O-])[C@@H](O)[C@H]3O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline diphosphate Identifier CHEBI:33019 (Beilstein: 185088) help_outline Charge -3 Formula HO7P2 InChIKeyhelp_outline XPPKVPWEQAFLFU-UHFFFAOYSA-K SMILEShelp_outline OP([O-])(=O)OP([O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 1,211 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:58136 | RHEA:58137 | RHEA:58138 | RHEA:58139 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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| MetaCyc help_outline |
Publications
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Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis.
Taniguchi T., Miyauchi K., Sakaguchi Y., Yamashita S., Soma A., Tomita K., Suzuki T.
Modification of tRNA anticodons plays a critical role in ensuring accurate translation. N<sup>4</sup>-acetylcytidine (ac<sup>4</sup>C) is present at the anticodon first position (position 34) of bacterial elongator tRNA<sup>Met</sup>. Herein, we identified Bacillus subtilis ylbM (renamed tmcAL) as ... >> More
Modification of tRNA anticodons plays a critical role in ensuring accurate translation. N<sup>4</sup>-acetylcytidine (ac<sup>4</sup>C) is present at the anticodon first position (position 34) of bacterial elongator tRNA<sup>Met</sup>. Herein, we identified Bacillus subtilis ylbM (renamed tmcAL) as a novel gene responsible for ac<sup>4</sup>C34 formation. Unlike general acetyltransferases that use acetyl-CoA, TmcAL activates an acetate ion to form acetyladenylate and then catalyzes ac<sup>4</sup>C34 formation through a mechanism similar to tRNA aminoacylation. The crystal structure of TmcAL with an ATP analog reveals the molecular basis of ac<sup>4</sup>C34 formation. The ΔtmcAL strain displayed a cold-sensitive phenotype and a strong genetic interaction with tilS that encodes the enzyme responsible for synthesizing lysidine (L) at position 34 of tRNA<sup>Ile</sup> to facilitate AUA decoding. Mistranslation of the AUA codon as Met in the ΔtmcAL strain upon tilS repression suggests that ac<sup>4</sup>C34 modification of tRNA<sup>Met</sup> and L34 modification of tRNA<sup>Ile</sup> act cooperatively to prevent misdecoding of the AUA codon. << Less
Nat. Chem. Biol. 14:1010-1020(2018) [PubMed] [EuropePMC]
This publication is cited by 2 other entries.
Comments
RHEA:58136 part of RHEA:58144