Reaction participants Show >> << Hide
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Namehelp_outline
a guanosine in mRNA
Identifier
RHEA-COMP:15584
Reactive part
help_outline
- Name help_outline GMP residue Identifier CHEBI:74269 Charge -1 Formula C10H11N5O7P SMILEShelp_outline C1(=O)NC(=NC2=C1N=CN2[C@@H]3O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]3O)N 2D coordinates Mol file for the small molecule Search links Involved in 42 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-methionine Identifier CHEBI:59789 Charge 1 Formula C15H23N6O5S InChIKeyhelp_outline MEFKEPWMEQBLKI-AIRLBKTGSA-O SMILEShelp_outline C[S+](CC[C@H]([NH3+])C([O-])=O)C[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12 2D coordinates Mol file for the small molecule Search links Involved in 842 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
an N7-methylguanosine in mRNA
Identifier
RHEA-COMP:15585
Reactive part
help_outline
- Name help_outline N7-methylguanosine 5'-phosphate residue Identifier CHEBI:74480 Charge 0 Formula C11H14N5O7P SMILEShelp_outline C1(=O)NC(=NC2=C1[N+](=CN2[C@@H]3O[C@H](COP(=O)(*)[O-])[C@@H](O*)[C@H]3O)C)N 2D coordinates Mol file for the small molecule Search links Involved in 8 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline S-adenosyl-L-homocysteine Identifier CHEBI:57856 Charge 0 Formula C14H20N6O5S InChIKeyhelp_outline ZJUKTBDSGOFHSH-WFMPWKQPSA-N SMILEShelp_outline Nc1ncnc2n(cnc12)[C@@H]1O[C@H](CSCC[C@H]([NH3+])C([O-])=O)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 768 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:60508 | RHEA:60509 | RHEA:60510 | RHEA:60511 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Related reactions help_outline
More general form(s) of this reaction
Publications
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Transcriptome-wide mapping of internal N7-methylguanosine methylome in mammalian mRNA.
Zhang L.S., Liu C., Ma H., Dai Q., Sun H.L., Luo G., Zhang Z., Zhang L., Hu L., Dong X., He C.
N<sup>7</sup>-methylguanosine (m<sup>7</sup>G) is a positively charged, essential modification at the 5' cap of eukaryotic mRNA, regulating mRNA export, translation, and splicing. m<sup>7</sup>G also occurs internally within tRNA and rRNA, but its existence and distribution within eukaryotic mRNA ... >> More
N<sup>7</sup>-methylguanosine (m<sup>7</sup>G) is a positively charged, essential modification at the 5' cap of eukaryotic mRNA, regulating mRNA export, translation, and splicing. m<sup>7</sup>G also occurs internally within tRNA and rRNA, but its existence and distribution within eukaryotic mRNA remain to be investigated. Here, we show the presence of internal m<sup>7</sup>G sites within mammalian mRNA. We then performed transcriptome-wide profiling of internal m<sup>7</sup>G methylome using m<sup>7</sup>G-MeRIP sequencing (MeRIP-seq). To map this modification at base resolution, we developed a chemical-assisted sequencing approach that selectively converts internal m<sup>7</sup>G sites into abasic sites, inducing misincorporation at these sites during reverse transcription. This base-resolution m<sup>7</sup>G-seq enabled transcriptome-wide mapping of m<sup>7</sup>G in human tRNA and mRNA, revealing distribution features of the internal m<sup>7</sup>G methylome in human cells. We also identified METTL1 as a methyltransferase that installs a subset of m<sup>7</sup>G within mRNA and showed that internal m<sup>7</sup>G methylation could affect mRNA translation. << Less