Rhea - reaction knowledgebase

Enzymes

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Name ^help_outline 2-oxoglutarate Identifier CHEBI:16810 (Beilstein: 3664503; CAS: 64-15-3) ^help_outline Charge -2 Formula C5H4O5 InChIKey^help_outline KPGXRSRHYNQIFN-UHFFFAOYSA-L SMILES^help_outline [O-]C(=O)CCC(=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 418 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline gibberellin A₁₂ Identifier CHEBI:58627 Charge -2 Formula C20H26O4 InChIKey^help_outline UJFQJDAESQJXTG-UFUZVNNQSA-L SMILES^help_outline [H][C@@]12CC[C@@H]3C[C@]1(CC3=C)[C@@H](C([O-])=O)[C@]1([H])[C@@](C)(CCC[C@@]21C)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 9 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline O₂ Identifier CHEBI:15379 (CAS: 7782-44-7) ^help_outline Charge 0 Formula O2 InChIKey^help_outline MYMOFIZGZYHOMD-UHFFFAOYSA-N SMILES^help_outline O=O 2D coordinates Mol file for the small molecule Search links Involved in 2,648 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline CO₂ Identifier CHEBI:16526 (Beilstein: 1900390; CAS: 124-38-9) ^help_outline Charge 0 Formula CO2 InChIKey^help_outline CURLTUGMZLYLDI-UHFFFAOYSA-N SMILES^help_outline O=C=O 2D coordinates Mol file for the small molecule Search links Involved in 980 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline gibberellin A₉ Identifier CHEBI:73255 Charge -1 Formula C19H23O4 InChIKey^help_outline MHVYWTXXZIFXDT-YGNOGLJPSA-M SMILES^help_outline [H][C@@]12C[C@]3(CC1=C)[C@@H](C([O-])=O)[C@]1([H])[C@@]4(C)CCC[C@@]1(OC4=O)[C@]3([H])CC2 2D coordinates Mol file for the small molecule Search links Involved in 5 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H₂O Identifier CHEBI:15377 (Beilstein: 3587155; CAS: 7732-18-5) ^help_outline Charge 0 Formula H2O InChIKey^help_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILES^help_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,048 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline succinate Identifier CHEBI:30031 (Beilstein: 1863859; CAS: 56-14-4) ^help_outline Charge -2 Formula C4H4O4 InChIKey^help_outline KDYFGRWQOYBRFD-UHFFFAOYSA-L SMILES^help_outline [O-]C(=O)CCC([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 325 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:60772	RHEA:60773	RHEA:60774	RHEA:60775
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	673 proteins	673 proteins

Publications

Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis.

Phillips A.L., Ward D.A., Uknes S., Appleford N.E.J., Lange T., Huttly A.K., Gaskin P., Graebe J.E., Hedden P.

Using degenerate oligonucleotide primers based on a pumpkin (Cucurbita maxima) gibberellin (GA) 20-oxidase sequence, six different fragments of dioxygenase genes were amplified by polymerase chain reaction from arabidopsis thaliana genomic DNA. One of these was used to isolate two different full-l ... >> More

Using degenerate oligonucleotide primers based on a pumpkin (Cucurbita maxima) gibberellin (GA) 20-oxidase sequence, six different fragments of dioxygenase genes were amplified by polymerase chain reaction from arabidopsis thaliana genomic DNA. One of these was used to isolate two different full-length cDNA clones, At2301 and At2353, from shoots of the GA-deficient Arabidopsis mutant ga1-2. A third, related clone, YAP169, was identified in the Database of Expressed Sequence Tags. The cDNA clones were expressed in Escherichia coli as fusion proteins, each of which oxidized GA12 at C-20 to GA15, GA24, and the C19 compound GA9, a precursor of bioactive GAs; the C20 tricarboxylic acid compound GA25 was formed as a minor product. The expression products also oxidized the 13-hydroxylated substrate GA53, but less effectively than GA12. The three cDNAs hybridized to mRNA species with tissue-specific patterns of accumulation, with At2301 being expressed in stems and inflorescences, At2353 in inflorescences and developing siliques, and YAP169 in siliques only. In the floral shoots of the ga1-2 mutant, transcript levels corresponding to each cDNA decreased dramatically after GA3 application, suggesting that GA biosynthesis may be controlled, at least in part, through down-regulation of the expression of the 20-oxidase genes. << Less

Plant Physiol. 108:1049-1057(1995) [PubMed] [EuropePMC]

This publication is cited by 6 other entries.

Comments

Published in: Toyomasu T., Kawaide H., Sekimoto H., von Numers C., Phillips A.L., Hedden P., Kamiya Y. Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings. Physiol. Plantarum 99:111-118(1997). DOI:10.1111/j.1399-3054.1997.tb03438.x Multistep reaction: RHEA:60776 + RHEA:60780 + RHEA:60788