Enzymes
UniProtKB help_outline | 489 proteins |
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Namehelp_outline
a 5'-end NAD+-phospho-ribonucleoside in snoRNA
Identifier
RHEA-COMP:15699
Reactive part
help_outline
- Name help_outline a 5'-end NAD+-phospho-ribonucleoside residue Identifier CHEBI:144029 Charge -2 Formula C26H32N7O20P3R SMILEShelp_outline N1(C2=C(C(=NC=N2)N)N=C1)[C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H]([N+]5=CC(=CC=C5)C(=O)N)[C@@H]([C@@H]4O)O)(=O)[O-])(=O)[O-])[C@H]([C@H]3O)OP(OC[C@H]6O[C@H]([C@@H]([C@@H]6O*)O)*)(=O)[O-] 2D coordinates Mol file for the small molecule Search links Involved in 3 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (Beilstein: 3587155; CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,048 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
a 5'-end phospho-ribonucleoside in snoRNA
Identifier
RHEA-COMP:15700
Reactive part
help_outline
- Name help_outline 5'-end ribonucleotide residue Identifier CHEBI:138282 Charge -2 Formula C5H7O7PR SMILEShelp_outline [C@@H]1(O[C@H]([C@@H]([C@@H]1O*)O)*)COP([O-])(=O)[O-] 2D coordinates Mol file for the small molecule Search links Involved in 27 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline NAD+ Identifier CHEBI:57540 (Beilstein: 3868403) help_outline Charge -1 Formula C21H26N7O14P2 InChIKeyhelp_outline BAWFJGJZGIEFAR-NNYOXOHSSA-M SMILEShelp_outline NC(=O)c1ccc[n+](c1)[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)n2cnc3c(N)ncnc23)[C@@H](O)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1,142 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:60892 | RHEA:60893 | RHEA:60894 | RHEA:60895 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
UniProtKB help_outline |
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Publications
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Eukaryotic RNA 5'-End NAD<sup>+</sup> Capping and DeNADding.
Kiledjian M.
A hallmark of eukaryotic mRNAs has long been the 5'-end m<sup>7</sup>G cap. This paradigm was recently amended by recent reports that Saccharomyces cerevisiae and mammalian cells also contain mRNAs carrying a novel nicotinamide adenine dinucleotide (NAD<sup>+</sup>) cap at their 5'-end. The presen ... >> More
A hallmark of eukaryotic mRNAs has long been the 5'-end m<sup>7</sup>G cap. This paradigm was recently amended by recent reports that Saccharomyces cerevisiae and mammalian cells also contain mRNAs carrying a novel nicotinamide adenine dinucleotide (NAD<sup>+</sup>) cap at their 5'-end. The presence of an NAD<sup>+</sup> cap on mRNA uncovers a previously unknown mechanism for controlling gene expression through nucleotide metabolite-directed mRNA turnover. In contrast to the m<sup>7</sup>G cap that stabilizes mRNA, the NAD<sup>+</sup> cap targets RNA for rapid decay in mammalian cells through the DXO non-canonical decapping enzyme which removes intact NAD<sup>+</sup> from RNA in a process termed 'deNADding'. This review highlights the identification of NAD<sup>+</sup> caps, their mode of addition, and their functional significance in cells. << Less
Trends Cell Biol 28:454-464(2018) [PubMed] [EuropePMC]
This publication is cited by 2 other entries.
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5' end nicotinamide adenine dinucleotide cap in human cells promotes RNA decay through DXO-mediated deNADding.
Jiao X., Doamekpor S.K., Bird J.G., Nickels B.E., Tong L., Hart R.P., Kiledjian M.
Eukaryotic mRNAs generally possess a 5' end N7 methyl guanosine (m<sup>7</sup>G) cap that promotes their translation and stability. However, mammalian mRNAs can also carry a 5' end nicotinamide adenine dinucleotide (NAD<sup>+</sup>) cap that, in contrast to the m<sup>7</sup>G cap, does not support ... >> More
Eukaryotic mRNAs generally possess a 5' end N7 methyl guanosine (m<sup>7</sup>G) cap that promotes their translation and stability. However, mammalian mRNAs can also carry a 5' end nicotinamide adenine dinucleotide (NAD<sup>+</sup>) cap that, in contrast to the m<sup>7</sup>G cap, does not support translation but instead promotes mRNA decay. The mammalian and fungal noncanonical DXO/Rai1 decapping enzymes efficiently remove NAD<sup>+</sup> caps, and cocrystal structures of DXO/Rai1 with 3'-NADP<sup>+</sup> illuminate the molecular mechanism for how the "deNADding" reaction produces NAD<sup>+</sup> and 5' phosphate RNA. Removal of DXO from cells increases NAD<sup>+</sup>-capped mRNA levels and enables detection of NAD<sup>+</sup>-capped intronic small nucleolar RNAs (snoRNAs), suggesting NAD<sup>+</sup> caps can be added to 5'-processed termini. Our findings establish NAD<sup>+</sup> as an alternative mammalian RNA cap and DXO as a deNADding enzyme modulating cellular levels of NAD<sup>+</sup>-capped RNAs. Collectively, these data reveal that mammalian RNAs can harbor a 5' end modification distinct from the classical m<sup>7</sup>G cap that promotes rather than inhibits RNA decay. << Less
Cell 168:1015-1027(2017) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.