Enzymes
UniProtKB help_outline | 2 proteins |
Enzyme class help_outline |
|
Reaction participants Show >> << Hide
- Name help_outline a (9Z,12Z)-octadecadienoyl-containing triacyl-sn-glycerol Identifier CHEBI:144774 Charge 0 Formula C23H36O6R2 SMILEShelp_outline O(C[C@@](COC(*)=O)(OC(=O)*)[H])C(*)=O 2D coordinates Mol file for the small molecule Search links Involved in 23 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline an N-(ω-hydroxy-ultra-long chain fatty acyl)-sphingoid base Identifier CHEBI:144784 Charge 0 Formula C5H10NO4R2 SMILEShelp_outline *[C@@H](O)[C@@H](NC(*CO)=O)CO 2D coordinates Mol file for the small molecule Search links Involved in 16 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline a diacylglycerol Identifier CHEBI:18035 Charge 0 Formula C5H6O5R2 SMILEShelp_outline [*]OCC(CO[*])O[*] 2D coordinates Mol file for the small molecule Search links Involved in 378 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline an N-[ω-(9Z,12Z-octadecadienoyloxy)-O-ultra-long chain fatty acyl]-sphingoid base Identifier CHEBI:144785 Charge 0 Formula C23H40NO5R2 SMILEShelp_outline *[C@@H](O)[C@@H](NC(*COC(CCCCCCC/C=C\C/C=C\CCCCC)=O)=O)CO 2D coordinates Mol file for the small molecule Search links Involved in 16 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:61528 | RHEA:61529 | RHEA:61530 | RHEA:61531 | |
---|---|---|---|---|
Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
UniProtKB help_outline |
|
|||
EC numbers help_outline | ||||
MetaCyc help_outline |
Related reactions help_outline
Specific form(s) of this reaction
Publications
-
PNPLA1 is a transacylase essential for the generation of the skin barrier lipid omega-O-acylceramide.
Ohno Y., Kamiyama N., Nakamichi S., Kihara A.
Lipids are the primary components of the skin permeability barrier, which is the body's most powerful defensive mechanism against pathogens. Acylceramide (ω-O-acylceramide) is a specialized lipid essential for skin barrier formation. Here, we identify PNPLA1 as the long-sought gene involved in the ... >> More
Lipids are the primary components of the skin permeability barrier, which is the body's most powerful defensive mechanism against pathogens. Acylceramide (ω-O-acylceramide) is a specialized lipid essential for skin barrier formation. Here, we identify PNPLA1 as the long-sought gene involved in the final step of acylceramide synthesis, esterification of ω-hydroxyceramide with linoleic acid, by cell-based assays. We show that increasing triglyceride levels by overproduction of the diacylglycerol acyltransferase DGAT2 stimulates acylceramide production, suggesting that triglyceride may act as a linoleic acid donor. Indeed, the in vitro analyses confirm that PNPLA1 catalyses acylceramide synthesis using triglyceride as a substrate. Mutant forms of PNPLA1 found in patients with ichthyosis exhibit reduced or no enzyme activity in either cell-based or in vitro assays. Altogether, our results indicate that PNPLA1 is directly involved in acylceramide synthesis as a transacylase, and provide important insights into the molecular mechanisms of skin barrier formation and of ichthyosis pathogenesis. << Less
Nat. Commun. 8:14610-14610(2017) [PubMed] [EuropePMC]
This publication is cited by 2 other entries.