Enzymes
| UniProtKB help_outline | 1 proteins |
Reaction participants Show >> << Hide
- Name help_outline an α-D-galactosaminyl-(1→3)-[α-L-fucosyl-(1→2)]-β-D-galactosyl derivative Identifier CHEBI:144802 Charge 1 Formula C18H33NO14R SMILEShelp_outline [C@@H]1([C@@H](O[C@@H]([C@@H]([C@@H]1O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)[NH3+])O)CO)O*)O[C@H]3[C@H]([C@@H]([C@@H]([C@@H](O3)C)O)O)O 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,648 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline D-galactosamine Identifier CHEBI:144817 Charge 1 Formula C6H14NO5 InChIKeyhelp_outline MSWZFWKMSRAUBD-GASJEMHNSA-O SMILEShelp_outline C1([C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)[NH3+])O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline an α-L-fucosyl-(1→2)-β-D-galactosyl derivative Identifier CHEBI:140327 Charge 0 Formula C12H21O10R SMILEShelp_outline [C@@H]1([C@@H](O[C@@H]([C@@H]([C@@H]1O)O)CO)O*)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:61568 | RHEA:61569 | RHEA:61570 | RHEA:61571 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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An enzymatic pathway in the human gut microbiome that converts A to universal O type blood.
Rahfeld P., Sim L., Moon H., Constantinescu I., Morgan-Lang C., Hallam S.J., Kizhakkedathu J.N., Withers S.G.
Access to efficient enzymes that can convert A and B type red blood cells to 'universal' donor O would greatly increase the supply of blood for transfusions. Here we report the functional metagenomic screening of the human gut microbiome for enzymes that can remove the cognate A and B type sugar a ... >> More
Access to efficient enzymes that can convert A and B type red blood cells to 'universal' donor O would greatly increase the supply of blood for transfusions. Here we report the functional metagenomic screening of the human gut microbiome for enzymes that can remove the cognate A and B type sugar antigens. Among the genes encoded in our library of 19,500 expressed fosmids bearing gut bacterial DNA, we identify an enzyme pair from the obligate anaerobe Flavonifractor plautii that work in concert to efficiently convert the A antigen to the H antigen of O type blood, via a galactosamine intermediate. The X-ray structure of the N-acetylgalactosamine deacetylase reveals the active site and mechanism of the founding member of an esterase family. The galactosaminidase expands activities within the CAZy family GH36. Their ability to completely convert A to O of the same rhesus type at very low enzyme concentrations in whole blood will simplify their incorporation into blood transfusion practice, broadening blood supply. << Less
Nat. Microbiol. 4:1475-1485(2019) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.