Reaction participants Show >> << Hide
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Namehelp_outline
7-cyano-7-carbaguanosine15 in tRNA
Identifier
RHEA-COMP:10371
Reactive part
help_outline
- Name help_outline 7-cyano-7-carbaguanine 5'-phosphate residue Identifier CHEBI:82850 Charge -1 Formula C12H11N5O7P Positionhelp_outline 15 SMILEShelp_outline Nc1nc2n(cc(C#N)c2c(=O)[nH]1)[C@@H]1O[C@H](COP([O-])(-*)=O)[C@@H](O-*)[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline L-lysine Identifier CHEBI:32551 Charge 1 Formula C6H15N2O2 InChIKeyhelp_outline KDXKERNSBIXSRK-YFKPBYRVSA-O SMILEShelp_outline [NH3+]CCCC[C@H]([NH3+])C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 67 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine15 in tRNA
Identifier
RHEA-COMP:16288
Reactive part
help_outline
- Name help_outline 7-N-[(5S)-5-amino-5-carboxypentyl]formamidino-7-deazaguanosine 5'-monophosphate residue Identifier CHEBI:145542 Charge 0 Formula C18H26N7O9P Positionhelp_outline 15 SMILEShelp_outline C1(=O)NC(=NC2=C1C(=CN2[C@@H]3O[C@H](COP(*)([O-])=O)[C@@H](O*)[C@H]3O)C(NCCCC[C@H]([NH3+])C(=O)[O-])=[NH2+])N 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:63216 | RHEA:63217 | RHEA:63218 | RHEA:63219 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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Identification of a radical SAM enzyme involved in the synthesis of archaeosine.
Yokogawa T., Nomura Y., Yasuda A., Ogino H., Hiura K., Nakada S., Oka N., Ando K., Kawamura T., Hirata A., Hori H., Ohno S.
Archaeosine (G<sup>+</sup>), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ<sub>0</sub>)-containing tRNA (q<sub>0</sub>N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (Arc ... >> More
Archaeosine (G<sup>+</sup>), 7-formamidino-7-deazaguanosine, is an archaea-specific modified nucleoside found at the 15th position of tRNAs. In Euryarchaeota, 7-cyano-7-deazaguanine (preQ<sub>0</sub>)-containing tRNA (q<sub>0</sub>N-tRNA), synthesized by archaeal tRNA-guanine transglycosylase (ArcTGT), has been believed to be converted to G<sup>+</sup>-containing tRNA (G<sup>+</sup>-tRNA) by the paralog of ArcTGT, ArcS. However, we found that several euryarchaeal ArcSs have lysine transfer activity to q<sub>0</sub>N-tRNA to form q<sub>0</sub>kN-tRNA, which has a preQ<sub>0</sub> lysine adduct as a base. Through comparative genomics and biochemical experiments, we found that ArcS forms a robust complex with a radical S-adenosylmethionine (SAM) enzyme named RaSEA. The ArcS-RaSEA complex anaerobically converted q<sub>0</sub>N-tRNA to G<sup>+</sup>-tRNA in the presence of SAM and lysine via q<sub>0</sub>kN-tRNA. We propose that ArcS and RaSEA should be considered an archaeosine synthase α-subunit (lysine transferase) and β-subunit (q<sub>0</sub>kN-tRNA lyase), respectively. << Less
Nat. Chem. Biol. 15:1148-1155(2019) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.