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- Name help_outline 6-phospho-N-acetyl-β-D-muramate-(1→4)-N-acetyl-D-glucosamine Identifier CHEBI:167477 Charge -3 Formula C19H30N2O16P InChIKeyhelp_outline XQLZNTOJYKUHKQ-NNRGKNABSA-K SMILEShelp_outline [C@H]1([C@H]([C@@H](O[C@@H]([C@H]1O)COP([O-])(=O)[O-])O[C@H]2[C@@H]([C@H](C(O[C@@H]2CO)O)NC(C)=O)O)NC(C)=O)O[C@@H](C(=O)[O-])C 2D coordinates Mol file for the small molecule Search links Involved in 2 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,485 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline N-acetyl-D-muramate 6-phosphate Identifier CHEBI:58722 Charge -3 Formula C11H17NO11P InChIKeyhelp_outline NMEMTQKUEVNSPV-MKFCKLDKSA-K SMILEShelp_outline C[C@@H](O[C@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)OC(O)[C@@H]1NC(C)=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 7 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline N-acetyl-D-glucosamine Identifier CHEBI:506227 (Beilstein: 1913592; CAS: 7512-17-6) help_outline Charge 0 Formula C8H15NO6 InChIKeyhelp_outline OVRNDRQMDRJTHS-RTRLPJTCSA-N SMILEShelp_outline CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 23 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:66780 | RHEA:66781 | RHEA:66782 | RHEA:66783 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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Recovery of the Peptidoglycan Turnover Product Released by the Autolysin Atl in Staphylococcus aureus Involves the Phosphotransferase System Transporter MurP and the Novel 6-phospho-N-acetylmuramidase MupG.
Kluj R.M., Ebner P., Adamek M., Ziemert N., Mayer C., Borisova M.
The peptidoglycan of the bacterial cell wall undergoes a permanent turnover during cell growth and differentiation. In the Gram-positive pathogen <i>Staphylococcus aureus</i>, the major peptidoglycan hydrolase Atl is required for accurate cell division, daughter cell separation and autolysis. Atl ... >> More
The peptidoglycan of the bacterial cell wall undergoes a permanent turnover during cell growth and differentiation. In the Gram-positive pathogen <i>Staphylococcus aureus</i>, the major peptidoglycan hydrolase Atl is required for accurate cell division, daughter cell separation and autolysis. Atl is a bifunctional <i>N</i>-acetylmuramoyl-L-alanine amidase/endo-β-<i>N</i>-acetylglucosaminidase that releases peptides and the disaccharide <i>N</i>-acetylmuramic acid-β-1,4-<i>N</i>-acetylglucosamine (MurNAc-GlcNAc) from the peptido-glycan. Here we revealed the recycling pathway of the cell wall turnover product MurNAc-GlcNAc in <i>S. aureus</i>. The latter disaccharide is internalized and concomitantly phosphorylated by the phosphotransferase system (PTS) transporter MurP, which had been implicated previously in the uptake and phosphorylation of MurNAc. Since MurP mutant cells accumulate MurNAc-GlcNAc and not MurNAc in the culture medium during growth, the disaccharide represents the physiological substrate of the PTS transporter. We further identified and characterized a novel 6-phospho-<i>N</i>-acetylmuramidase, named MupG, which intracellularly hydrolyses MurNAc 6-phosphate-GlcNAc, the product of MurP-uptake and phosphorylation, yielding MurNAc 6-phosphate and GlcNAc. MupG is the first characterized representative of a novel family of glycosidases containing domain of unknown function 871 (DUF871). The corresponding gene <i>mupG</i> (<i>SAUSA300_0192</i>) of <i>S. aureus</i> strain USA300 is the first gene within a putative operon that also includes genes encoding the MurNAc 6-phosphate etherase MurQ, MurP, and the putative transcriptional regulator MurR. Using mass spectrometry, we observed cytoplasmic accumulation of MurNAc 6-phosphate-GlcNAc in Δ<i>mupG</i> and Δ<i>mupGmurQ</i> markerless non-polar deletion mutants, but not in the wild type or in the complemented Δ<i>mupG</i> strain. MurNAc 6-phosphate-GlcNAc levels in the mutants increased during stationary phase, in accordance with previous observations regarding peptidoglycan recycling in <i>S. aureus</i>. << Less
Front. Microbiol. 9:2725-2725(2018) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.