Enzymes
| UniProtKB help_outline | 1 proteins |
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- Name help_outline dihydroxyacetone phosphate Identifier CHEBI:57642 (Beilstein: 4428349) help_outline Charge -2 Formula C3H5O6P InChIKeyhelp_outline GNGACRATGGDKBX-UHFFFAOYSA-L SMILEShelp_outline C(CO)(COP([O-])(=O)[O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 41 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline L-glutamate Identifier CHEBI:29985 (CAS: 11070-68-1) help_outline Charge -1 Formula C5H8NO4 InChIKeyhelp_outline WHUUTDBJXJRKMK-VKHMYHEASA-M SMILEShelp_outline [NH3+][C@@H](CCC([O-])=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 242 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline (S)-serinol phosphate Identifier CHEBI:184377 Charge -1 Formula C3H9NO5P InChIKeyhelp_outline WDWYJNPKBKWDBL-VKHMYHEASA-M SMILEShelp_outline [C@H](CO)(COP([O-])(=O)[O-])[NH3+] 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 2-oxoglutarate Identifier CHEBI:16810 (Beilstein: 3664503; CAS: 64-15-3) help_outline Charge -2 Formula C5H4O5 InChIKeyhelp_outline KPGXRSRHYNQIFN-UHFFFAOYSA-L SMILEShelp_outline [O-]C(=O)CCC(=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 418 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:69552 | RHEA:69553 | RHEA:69554 | RHEA:69555 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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Functional Characterization of Two PLP-Dependent Enzymes Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.
Riegert A.S., Narindoshvili T., Coricello A., Richards N.G.J., Raushel F.M.
<i>Campylobacter jejuni</i> is a Gram-negative, pathogenic bacterium that causes campylobacteriosis, a form of gastroenteritis. <i>C. jejuni</i> is the most frequent cause of food-borne illness in the world, surpassing <i>Salmonella</i> and <i>E. coli</i>. Coating the surface of <i>C. jejuni</i> i ... >> More
<i>Campylobacter jejuni</i> is a Gram-negative, pathogenic bacterium that causes campylobacteriosis, a form of gastroenteritis. <i>C. jejuni</i> is the most frequent cause of food-borne illness in the world, surpassing <i>Salmonella</i> and <i>E. coli</i>. Coating the surface of <i>C. jejuni</i> is a layer of sugar molecules known as the capsular polysaccharide that, in <i>C. jejuni</i> NCTC 11168, is composed of a repeating unit of d-glycero-l-gluco-heptose, d-glucuronic acid, d-<i>N</i>-acetyl-galactosamine, and d-ribose. The d-glucuronic acid moiety is further amidated with either serinol or ethanolamine. It is unknown how these modifications are synthesized and attached to the polysaccharide. Here, we report the catalytic activities of two previously uncharacterized, pyridoxal phosphate (PLP)-dependent enzymes, Cj1436 and Cj1437, from <i>C. jejuni</i> NCTC 11168. Using a combination of mass spectrometry and nuclear magnetic resonance, we determined that Cj1436 catalyzes the decarboxylation of l-serine phosphate to ethanolamine phosphate. Cj1437 was shown to catalyze the transamination of dihydroxyacetone phosphate to (<i>S</i>)-serinol phosphate in the presence of l-glutamate. The probable routes to the ultimate formation of the glucuronamide substructures in the capsular polysaccharides of <i>C. jejuni</i> are discussed. << Less
Biochemistry 60:2836-2843(2021) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.