Enzymes
| UniProtKB help_outline | 4,334 proteins |
Reaction participants Show >> << Hide
- Name help_outline guanidine Identifier CHEBI:30087 (Beilstein: 1902006) help_outline Charge 1 Formula CH6N3 InChIKeyhelp_outline ZRALSGWEFCBTJO-UHFFFAOYSA-O SMILEShelp_outline NC(N)=[NH2+] 2D coordinates Mol file for the small molecule Search links Involved in 5 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:73883 | RHEA:73884 | RHEA:73885 | RHEA:73886 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Interaction of cations, anions, and weak base quinine with rat renal cation transporter rOCT2 compared with rOCT1.
Arndt P., Volk C., Gorboulev V., Budiman T., Popp C., Ulzheimer-Teuber I., Akhoundova A., Koppatz S., Bamberg E., Nagel G., Koepsell H.
The rat organic cation transporter (rOCT)-2 was characterized by electrical and tracer flux measurements compared with rOCT1. By applying choline gradients to voltage-clamped Xenopus oocytes expressing rOCT2, potential-dependent currents could be induced in both directions. Tracer flux measurement ... >> More
The rat organic cation transporter (rOCT)-2 was characterized by electrical and tracer flux measurements compared with rOCT1. By applying choline gradients to voltage-clamped Xenopus oocytes expressing rOCT2, potential-dependent currents could be induced in both directions. Tracer flux measurements with seven organic cations revealed similar Michaelis-Menten constant values for both transporters, with the exception of guanidine. In parallel experiments with rOCT2 and rOCT1, inhibition of tetraethylammonium transport by 12 cations, 2 weak bases, corticosterone, and the anions para-amminohippurate, alpha-ketoglutarate, and probenecid was characterized. The IC(50) values of many inhibitors were similar for both transporters, whereas others were significantly different. Mepiperphenidol and O-methylisoprenaline showed an approximately 70-fold lower and corticosterone a 38-fold higher affinity for rOCT2. With the use of these inhibitors together with previous information on cation transporters, experimental protocols are proposed to dissect out the individual contributions of rOCT2 and rOCT1 in intact proximal tubule preparations. Inhibition experiments at different pH levels strongly suggest that the weak base quinine passively permeates the plasma membrane at physiological pH and inhibits rOCT2 from the intracellular side. << Less
Am. J. Physiol. 281:F454-F468(2001) [PubMed] [EuropePMC]
This publication is cited by 3 other entries.