Enzymes
| UniProtKB help_outline | 41 proteins |
Reaction participants Show >> << Hide
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Namehelp_outline
a 3ʼ-end 2ʼ-deoxyribonucleotide-3ʼ-phosphoglycolate-DNA
Identifier
RHEA-COMP:19686
Reactive part
help_outline
- Name help_outline a 3ʼ-end deoxyribonucleotide 3ʼ-phosphoglycolate residue Identifier CHEBI:231894 Charge -3 Formula C7H9O10P2R SMILEShelp_outline *[C@@H]1O[C@H](COP(*)(=O)[O-])[C@H](C1)OP(OCC(=O)[O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,485 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 2-phosphoglycolate Identifier CHEBI:58033 Charge -3 Formula C2H2O6P InChIKeyhelp_outline ASCFNMCAHFUBCO-UHFFFAOYSA-K SMILEShelp_outline [O-]C(=O)COP([O-])([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 3 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
a 3'-end 2'-deoxyribonucleotide-DNA
Identifier
RHEA-COMP:13863
Reactive part
help_outline
- Name help_outline 3'-end deoxyribonucleotide residue Identifier CHEBI:138148 Charge -1 Formula C5H8O5PR SMILEShelp_outline *[C@@H]1O[C@H](COP(*)(=O)[O-])[C@H](C1)O 2D coordinates Mol file for the small molecule Search links Involved in 5 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,932 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:81467 | RHEA:81468 | RHEA:81469 | RHEA:81470 | |
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| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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| EcoCyc help_outline |
Publications
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Removal of 3'-phosphoglycolate from DNA strand-break damage in an oligonucleotide substrate by recombinant human apurinic/apyrimidinic endonuclease 1.
Winters T.A., Henner W.D., Russell P.S., McCullough A., Jorgensen T.J.
A recombinant human AP endonuclease, HAP1, was constructed and characterized with respect to its ability to recognize and act upon a model double-stranded 39-mer oligodeoxyribonucleotide substrate containing a strand break site with 3'-phosphoglycolate and 5'-phosphate end-group chemistries. This ... >> More
A recombinant human AP endonuclease, HAP1, was constructed and characterized with respect to its ability to recognize and act upon a model double-stranded 39-mer oligodeoxyribonucleotide substrate containing a strand break site with 3'-phosphoglycolate and 5'-phosphate end-group chemistries. This oligodeoxyribonucleotide substrate exactly duplicates the chemistry and configuration of a major DNA lesion produced by ionizing radiation. HAP1 was found to recognize the strand break, and catalyze the release of the 3'-phosphoglycolate as free phosphoglycolic acid. The enzyme had a Vmax of 0.1 fmole/min/pg of HAP1 protein, and a Km of 0.05 microM for the 3'-phosphoglycolate strand break lesion. The mechanism of catalysis was hydrolysis of the phosphate ester bond between the 3'-phosphoglycolate moiety and the 3'-carbon of the adjacent dGMP moiety within the oligonucleotide. The resulting DNA contained a 3'-hydroxyl which supported nucleotide incorporation by E. coli DNA polymerase I large fragment. AP endonucleolytic activity of HAP1 was examined using an analogous double-stranded 39-mer oligodeoxyribonucleotide substrate, in which the strand break site was replaced by an apyrimidinic site. The Vmax and Km for the AP endonuclease reaction were 68 fmole/min/pg of HAP1 protein and 0.23 microM, respectively. << Less