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Namehelp_outline
a 3ʼ-end 2ʼ-deoxyribonucleotide-8-oxoguanine-DNA
Identifier
RHEA-COMP:19687
Reactive part
help_outline
- Name help_outline a 3ʼ-end deoxyribonucleotide-8-oxoguanine residue Identifier CHEBI:231896 Charge -2 Formula C15H19N5O12P2R SMILEShelp_outline *[C@@H]1O[C@H](COP(*)(=O)[O-])[C@H](C1)OP(OC[C@H]2O[C@@H](C[C@@H]2O)N3C4=C(NC3=O)C(N=C(N4)N)=O)([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,485 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline 8-oxo-dGMP Identifier CHEBI:63224 Charge -2 Formula C10H12N5O8P InChIKeyhelp_outline AQIVLFLYHYFRKU-VPENINKCSA-L SMILEShelp_outline Nc1nc2n([C@H]3C[C@H](O)[C@@H](COP([O-])([O-])=O)O3)c(=O)[nH]c2c(=O)[nH]1 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
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Namehelp_outline
a 3'-end 2'-deoxyribonucleotide-DNA
Identifier
RHEA-COMP:13863
Reactive part
help_outline
- Name help_outline 3'-end deoxyribonucleotide residue Identifier CHEBI:138148 Charge -1 Formula C5H8O5PR SMILEShelp_outline *[C@@H]1O[C@H](COP(*)(=O)[O-])[C@H](C1)O 2D coordinates Mol file for the small molecule Search links Involved in 5 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,932 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:81471 | RHEA:81472 | RHEA:81473 | RHEA:81474 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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APE1-dependent repair of DNA single-strand breaks containing 3'-end 8-oxoguanine.
Parsons J.L., Dianova I.I., Dianov G.L.
DNA single-strand breaks containing 3'-8-oxoguanine (3'-8-oxoG) ends can arise as a consequence of ionizing radiation and as a result of DNA polymerase infidelity by misincorporation of 8-oxodGMP. In this study we examined the mechanism of repair of 3'-8-oxoG within a single-strand break using pur ... >> More
DNA single-strand breaks containing 3'-8-oxoguanine (3'-8-oxoG) ends can arise as a consequence of ionizing radiation and as a result of DNA polymerase infidelity by misincorporation of 8-oxodGMP. In this study we examined the mechanism of repair of 3'-8-oxoG within a single-strand break using purified base excision repair enzymes and human whole cell extracts. We find that 3'-8-oxoG inhibits ligation by DNA ligase IIIalpha or DNA ligase I, inhibits extension by DNA polymerase beta and that the lesion is resistant to excision by DNA glycosylases involved in the repair of oxidative lesions in human cells. However, we find that purified human AP-endonuclease 1 (APE1) is able to remove 3'-8-oxoG lesions. By fractionation of human whole cell extracts and immunoprecipitation of fractions containing 3'-8-oxoG excision activity, we further demonstrate that APE1 is the major activity involved in the repair of 3'-8-oxoG lesions in human cells and finally we reconstituted repair of the 3'-8-oxoG-containing oligonucleotide duplex with purified human enzymes including APE1, DNA polymerase beta and DNA ligase IIIalpha. << Less