Reaction participants Show >> << Hide
- Name help_outline an α-L-Fuc-(1→2)-β-D-Gal-(1→4)-β-D-GlcNAc derivative Identifier CHEBI:133510 Charge 0 Formula C20H34NO15R SMILEShelp_outline O([C@H]1[C@@H]([C@H]([C@@H](O[C@@H]1CO)O*)NC(C)=O)O)[C@@H]2O[C@@H]([C@H](O)[C@H](O)[C@H]2O[C@@H]3O[C@H]([C@H]([C@H]([C@@H]3O)O)O)C)CO 2D coordinates Mol file for the small molecule Search links Involved in 4 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H2O Identifier CHEBI:15377 (CAS: 7732-18-5) help_outline Charge 0 Formula H2O InChIKeyhelp_outline XLYOFNOQVPJJNP-UHFFFAOYSA-N SMILEShelp_outline [H]O[H] 2D coordinates Mol file for the small molecule Search links Involved in 6,485 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative Identifier CHEBI:133507 Charge 0 Formula C14H24NO11R SMILEShelp_outline [C@@H]1([C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O)O[C@H]2[C@@H]([C@H]([C@@H](O[C@@H]2CO)O*)NC(=O)C)O 2D coordinates Mol file for the small molecule Search links Involved in 12 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline L-fucose Identifier CHEBI:2181 (CAS: 2438-80-4) help_outline Charge 0 Formula C6H12O5 InChIKeyhelp_outline SHZGCJCMOBCMKK-DHVFOXMCSA-N SMILEShelp_outline C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 15 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
| RHEA:82807 | RHEA:82808 | RHEA:82809 | RHEA:82810 | |
|---|---|---|---|---|
| Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
| UniProtKB help_outline |
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Publications
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Turning universal O into rare Bombay type blood.
Anso I., Naegeli A., Cifuente J.O., Orrantia A., Andersson E., Zenarruzabeitia O., Moraleda-Montoya A., Garcia-Alija M., Corzana F., Del Orbe R.A., Borrego F., Trastoy B., Sjogren J., Guerin M.E.
Red blood cell antigens play critical roles in blood transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency in H antigen, the O<sub>h</sub> Bombay phenotype, can only be transfused with group O<sub>h</sub> blood to avoid serious transfusion reactions. We ... >> More
Red blood cell antigens play critical roles in blood transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency in H antigen, the O<sub>h</sub> Bombay phenotype, can only be transfused with group O<sub>h</sub> blood to avoid serious transfusion reactions. We discover FucOB from the mucin-degrading bacteria Akkermansia muciniphila as an α-1,2-fucosidase able to hydrolyze Type I, Type II, Type III and Type V H antigens to obtain the afucosylated Bombay phenotype in vitro. X-ray crystal structures of FucOB show a three-domain architecture, including a GH95 glycoside hydrolase. The structural data together with site-directed mutagenesis, enzymatic activity and computational methods provide molecular insights into substrate specificity and catalysis. Furthermore, using agglutination tests and flow cytometry-based techniques, we demonstrate the ability of FucOB to convert universal O type into rare Bombay type blood, providing exciting possibilities to facilitate transfusion in recipients/patients with Bombay phenotype. << Less
Nat Commun 14:1765-1765(2023) [PubMed] [EuropePMC]
This publication is cited by 1 other entry.